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huPBMC-NKG-MHC-dKO Mouse
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huPBMC-NKG-MHC-dKO Mouse
Product Name
huPBMC-NKG-MHC-dKO Mouse
Product ID
C002134
Strain Name
huPBMC-NOD.Cg-PrkdcscidIl2rgem1cyaH2-K1em1cyaH2-Ab1em2cyaH2-D1em1cya/Cya
Background
NKG
Status
Citations
0
When using this mouse strain in a publication, please cite “huPBMC-NKG-MHC-dKO Mouse (Catalog C002134) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
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Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Basic Information
Related Resource
Basic Information
Gene Name
H2-Ab1 & H2-D1 & H2-K1 & Il2rg
Gene Alias
IAb, Ia2, Ia-2, Abeta, H-2Ab, H2-Ab, Rmcs1, I-Abeta, H-2D, H2-D, H2-K1, K-f, H-2K, H2-K, H2-D1, H-2K(d), gc, p64, [g]c, CD132, gamma(c)
NCBI ID
Chromosome
Chr 17 (Mouse), Chr 17 (Mouse), Chr 17 (Mouse), Chr X (Mouse)
MGI ID
Datasheet
Strain Description
NKG mice (Catalog No.: C001316) are a type of severely immunodeficient mouse independently developed by Cyagen through knockout of the Il2rg gene on the NOD-Scid background. This strain lacks mature T, B, and NK cells, has reduced complement activity, and its macrophages exhibit weak phagocytosis of human cells. Therefore, NKG mice can efficiently engraft human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), patient-derived tumor xenografts (PDX), or adult stem cells and tissues.
A series of molecules encoded by the mouse major histocompatibility complex (MHC) play a central role in antigen processing and T cell-mediated immune responses. These molecules are roughly divided into two classes: class I molecules (e.g., H2-K1 and H2-D1), which are widely expressed on the surface of all nucleated cells and primarily function to present endogenous antigens to CD8+ cytotoxic T cells; and class II molecules (e.g., H2-Ab1), which are mainly expressed by professional antigen-presenting cells (APCs), including macrophages, B cells, and dendritic cells, and are responsible for presenting exogenous antigens to CD4+ helper T cells [1-2].
Previous studies have shown that double knockout (dKO) of MHC class I and class II molecules in mice can block the xenogeneic antigen presentation process, thereby alleviating graft-versus-host disease (GvHD) and enabling long-term engraftment of human immune cells [3-5]. NKG-MHC-dKO(H2-K1/H2-Ab1/H2-D1) mice (Catalog No.: C001705), generated by targeted deletion of the key MHC class I molecules (H2-K1 and H2-D1) and the class II molecule (H2-Ab1), can circumvent the immune mismatch that triggers GvHD.
The huPBMC-NKG-MHC-dKO mouse is an immune system-humanized model constructed by transplanting human PBMC into NKG-MHC-dKO(H2-K1/H2-Ab1/H2-D1) mice (Catalog No.: C001705). This model may help reduce GvHD associated with immune mismatch after PBMC transplantation and is suitable for long-term in vivo research and evaluation of human immune system reconstitution.
Reference
Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System in Health and Disease. 5th edition. New York: Garland Science; 2001. The major histocompatibility complex and its functions.
Shiina T, Blancher A, Inoko H, Kulski JK. Comparative genomics of the human, macaque and mouse major histocompatibility complex. Immunology. 2017 Feb;150(2):127-138.
Yaguchi T, Kobayashi A, Inozume T, Morii K, Nagumo H, Nishio H, Iwata T, Ka Y, Katano I, Ito R, Ito M, Kawakami Y. Human PBMC-transferred murine MHC class I/II-deficient NOG mice enable long-term evaluation of human immune responses. Cell Mol Immunol. 2018 Nov;15(11):953-962.
Cogels MM, Rouas R, Ghanem GE, Martinive P, Awada A, Van Gestel D, Krayem M. Humanized Mice as a Valuable Pre-Clinical Model for Cancer Immunotherapy Research. Front Oncol. 2021 Nov 18;11:784947.
Brehm MA, Kenney LL, Wiles MV, Low BE, Tisch RM, Burzenski L, Mueller C, Greiner DL, Shultz LD. Lack of acute xenogeneic graft- versus-host disease, but retention of T-cell function following engraftment of human peripheral blood mononuclear cells in NSG mice deficient in MHC class I and II expression. FASEB J. 2019 Mar;33(3):3137-3151.
Application Area
For in vivo studies that require mature T cells in areas such as tumor immunity, GvHD, hematological diseases, and infectious diseases (e.g., HIV/AIDS), thereby facilitating the development of new drugs related to immunotherapy.
Related Resource
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