|NCBI Gene ID||20351|
|Genetic Modification||Conventional knockout|
|Description||The Sema4a gene (NCBI Reference Sequence: NM_013658.3; Ensembl: ENSMUSG00000028064) is located on mouse chromosome 3. Fifteen exons are identified, with the ATG start codon in exon 2 and the TAA stop codon in exon 15 (Transcript Sema4a- 201: ENSMUST00000029700). Exon 3~10 will be selected as target site. Cas9 and gRNA will be co-injected into fertilized eggs for KO mouse production.|
|Quality Control||Sperm Quality Evaluation||Pre-cryopreservation: Measurement of sperm concentration, determination of sperm
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
|Product Information||Source||Cryopreserved sperm|
|Strategy Download||Inquire Now|
Cyagen Knockout Catalog Models repository provides a searchable listing of over 16k custom KO and cKO/floxed strains from which live mice are available to researchers in as few as two(2) weeks. Our repository is constantly growing, so feel free to inquire about the status of any specific gene(s) not shown online for more information about pending availability.