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- ● Diet-induced obesity (DIO) Model
- ● Type 1 Diabetes Mellitus (T1DM) Mouse Model
- ● Type 2 Diabetes Mellitus (T2DM) Mouse Model
- ● Diet-induced Non-alcoholic Fatty Liver Disease (NAFLD) Mouse Model
- ● Chemically Induced Non-alcoholic Fatty Liver Disease (NAFLD) Mouse Model
- ● Non-alcoholic Fatty Liver Disease (NAFLD) Mouse Model (Gene-editing)
- ● Composite (Combined) Model - Non-alcoholic Fatty Liver Disease (NAFLD) Model
- ● Composite Model - Atherosclerosis Mouse Model
- ● Atherosclerosis Mouse Model (Gene-editing)
- ● Acute Pancreatitis Mouse Model
- ● Chronic Pancreatitis Mouse Model
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Adeno-associated Virus Packaging
Description
Adeno-associated Virus (AAVs) is currently the most widely used delivery vector in gene transduction and gene therapy due to its advantages of good tissue specificity, low immunogenicity, and high safety. It is also a powerful tool in regenerative medicine due to its broad tropism and ability to transduce both proliferating and quiescent cells. Cyagen provides premium AAV packaging services to support your AAV mediated gene therapy research. For AAV virus packaging, we adopt a three-plasmid co-transfection method. The advanced purification process can provide customized AAV packaging services with high purity, high titer, and different serotypes. Our AAV production services are especially suitable for in vivo animal experiments and can meet the personalized choices of gene therapy researchers.
Service Details
Cyagen can provide the following AAV packaging services (and more upon request):
|
AAV Type |
Vector Production Scale |
Virus amount |
Titer |
Turnaround |
|
Over-expression |
Purified pilot |
≥1*10^12 GC |
≥1*10^12 GC/mL |
5~6 weeks |
|
Purified medium |
≥5*10^12 GC |
≥1*10^13 GC/mL |
||
|
Interference |
Purified pilot(3+1) |
≥1*10^12 GC |
≥1*10^12 GC/mL |
|
|
Purified medium(3+1) |
≥5*10^12 GC |
≥1*10^13 GC/mL |
||
|
Knock-out |
Purified pilot |
≥1*10^12 GC |
≥1*10^12 GC/mL |
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|
Purified medium |
≥5*10^12 GC |
≥1*10^13 GC/mL |
Alternative serotypes:
|
Serotype |
Target tissue |
Serotype |
Target tissue |
|
AAV1 |
muscles, heart, nerves, skeletal muscles |
AAV rh10 |
liver, blood, heart, extracorporeal cells |
|
AAV2 |
muscles, liver, brain tissue, eyes, nerves |
AAV2-retro |
nerve cell (reverse non-transsynaptic) |
|
AAV5 |
lungs, eyes, nerves, pancreas |
AAV-DJ |
retina, lung, kidney, cells in vitro |
|
AAV6 |
lungs, heart |
AAV-DJ-8 |
liver, eyes, central nervous system |
|
AAV7 |
muscle, liver |
AAV9-CNS |
central nervous system |
|
AAV8 |
muscles, liver, nerves, eyes |
AAV9-PNS |
peripheral nervous system |
|
AAV9 |
heart, muscles, lungs, liver, nerves |
More serotypes |
|
AAV Production Process
Advantages of Cyagen’s AAV packaging service
- Short cycle
Standard projects shipped in as fast as two weeks, customized projects can be done in as fast as five weeks (additional time for delivery). - Reliable quality
The virus titer is guaranteed to be ≥1*1012 GC/mL, the purity is ≥95%, and the endotoxin is less than 10 EU/mL, ensuring high infection efficiency. - Professional support
Our experts provide technical guidance for the entire AAV production process: from vector design, virus packaging, and in vivo injection to phenotypic analysis. - Prolific experience
More than ten years of virus packaging experience, continuously expanding stock library, and numerous customer literature citations and publications.
Artificial Intelligence (AI) Bioinformatics Platform
Our experienced artificial intelligence (AI) and bioinformatics team provide cutting-edge AAV carrier development, optimization, and transformation services.
Inquiries and Quote Requests
Request a quote now. Alternatively, you can always email cell-service@cyagen.com to inquire about our services or obtain a quote for your project.
