TurboKnockout® Gene Targeting Mice

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knockout mouse models |  Turboknockout conditional knockout mice (floxed mice) - Cyagen US Inc.

Get Your Conditional Knockout & Knockin Mice in as Fast as 6 Months!

Our new TurboKnockout® service can provide you with conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months. This proprietary gene targeting method is enabled by two innovations:
(1) A super competent ES cell line that generates 100% ESC-derived founder mice rather than chimeras, eliminating the need to screen for germline transmission.
(2) A selection cassette on the targeting construct capable of removing itself after ES cell targeting without the need to breed to Flp deleter mice.
These innovations eliminate two generations of breeding, shortening production time by 4-6 months as compared to industry standard. For conditional knockouts, it may be possible to breed founders directly to your tissue-specific Cre mice.
All projects are backed by our full money-back guarantee - no animals, no charge!


Knockout Mouse Catalog Models: Over 10,000 KO Strains, Delivered as Fast as 3 Months

ACE2 Humanized Mouse Models: As Fast As 2 Months

Taconic-Cyagen Custom Animal Awards: Win a Free Mouse Model

◆ Workflow of TurboKnockout® Gene Targeting Mouse Services

Each full gene targeting project is split into the following phases:


Workflow of knockout mouse service | Turboknockout conditional knockout mice - Cyagen US Inc.



1. TurboKnockout® gene targeting strategy design

  • Analyze relevant information for your target gene, including gene structure, neighboring genes at the genomic locus, known targeted animal models, etc.
  • Develop a gene targeting strategy, including the method for screening targeted ES cells by PCR and confirmation by Southern blot.
  • Propose the strategy to you for review and approval.


2. TurboKnockout® targeting vector construction

  • Clone relevant DNA fragments corresponding to homology arms into the targeting vector.
  • Confirm construction of the final targeting vector by restriction digest and sequencing.


3. Targeting in ES cells

  • Electroporate targeting vector into our super competent TurboKnockout® ES cells, followed by appropriate drug selection and isolation of drug-resistant clones. By default, our ES cells are derived from the C57BL/6 strain. Additionally, we offer services using BALB/c-derived ES cells.
  • Screen for targeted ES cell clones by PCR.
  • Expand up to six PCR-positive clones, which are further confirmed by Southern blot.
  • Karyotype ES cells to ensure correct chromosome number.


4. TurboKnockout® mouse production

  • Introduce targeted ES cells into host embryos, followed by transfer into surrogate mothers. The resulting TurboKnockout® heterozygous mutants are 100% derived from the injected ES cells. We will aim to generate a minimum of 3 mice.
  • Genotype founders by PCR to confirm their mutant status.
  • Optional: Breed to F1.
  • Delivery of mice to the customer.


 Types of Knockout Mouse Services 

Conventional knockout

In conventional knockout models, one or more critical exons of the target gene are replaced with a drug-selection cassette. This results in the permanent inactivation of the target gene in all cells of the body throughout development.types of knockout mice: conventional knockout mice - Cyagen US Inc.


Conditional knockout

In conditional knockout models, one or more critical exons of the target gene are flanked by LoxP sequences which can be recognized by Cre recombinase. By breeding these floxed mice (mice with gene locus flanked by LoxP) with tissue-specific Cre-expressing mice (Cre deleters), it is possible to specifically delete the floxed region and inactivate the gene in desired tissues, while in all other tissues, the target gene remains functional. types of knockout mice: Conditional knockout mice - Cyagen US Inc.



- Reporter knockinA visually detectable marker gene (such as GFP or lacZ) is knocked into a gene locus to replace the coding sequence of the target gene for the purpose of monitoring the promoter activity of the gene. Alternatively, a marker or an epitope tag can be knocked into the end (or less frequently, the beginning) of the coding sequence to form a fusion protein, which allows target gene expression and localization/trafficking to be examined.

 types of knockout mice: knockin mice



- Point mutation knockinBy introducing point mutation(s) into homology arms or the targeting vector, desired mutation(s) can be incorporated into the target gene. The mutated gene is expressed under the control of the wildtype gene regulatory elements. 

types of knockout mice: point mutation knockin mice


- Humanized mouseA humanized mouse is defined as a mouse model in which a portion or the full mouse gene sequence has been replaced by its human counterpart. The human gene is expressed under the control of the wildtype mouse regulatory sequences.

types of knockout mice: humanized mice - Cyagen US Inc.


- ROSA26 knockin: ROSA26 is a locus that has been widely used for achieving global expression of the introduced gene in mice. The gene of interest is targeted into the first intron of this locus. The expression of the gene can be driven by the endogenous ROSA26 promoter or by a promoter of choice that is targeted into the locus along with the gene. 





◆ Pricing and Turnaround Time

Service Price (Standard B6 strains) Turnaround time
TurboKnockout® targeting strategy design Free 1-4 days
Construction of TurboKnockout® gene targeting vector $8,950 8-14 weeks
Gene targeting in ES cells $12,950 8-14 weeks
Karyotype ES cells to ensure correct chromosome number $350/clone 1-2 weeks
TurboKnockout® mouse production $8,950
(we will inject as many ES cell clones as needed to fulfill our guarantee)
8-12 weeks
Optional: Breeding founders to obtain F1 Please inquire*

* You can choose to receive F0 founder animals and perform their own breeding to F1 mice. To ensure the successful generation of F1 mice and germline transmission, breeding of F0 animals require adherence to Turboknockout® breeding protocol, which will be supplied to you. You can also choose to have Cyagen perform F1 breeding, which adds 1-3 months to the projected turnaround time. You can choose to breed F0 founders to wildtype mice or to other stains such as Cre deleter strains of your choice; additional service fees may apply.


Note: For special knockout/knockin services not listed above, please inquire about availability and pricing. The turnaround time above does not include the time for obtaining host institution’s approval for mouse importation and transit time during shipping.


Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.

◆ Inquiries and Quote Requests

Request a quote now. Alternatively, you can always email animal-service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.

◆ Price Matching

If you find another service provider that offers better pricing than ours, we will match the price plus an additional 5% off.

◆ Payments

Standard payment terms include a 50% upfront payment before the project begins, and the remaining 50% plus shipping paid after completion of the project. If you need us to design your targeting strategy, we will provide this service for free irrespective of whether you end up choosing us for your project (other companies typically charge a few thousand dollars for this service).

◆ Bulk Discount

We offer up to a 10% bulk discount for large orders. Large orders are defined as 5 or more projects from the same institution. If you bundle your orders with those of your colleagues, you can all qualify for the bulk discount.

◆ Shipping

Products are shipped from our facility in China to our Santa Clara, California facility, then are relayed to end users. For mouse shipments, the shipping charge includes courier cost plus a $100/crate handling fee. Cells are shipped on dry ice, and the charge includes courier cost plus a $45 handling fee. DNA constructs are shipped in E. coli at room temperature, and the charge includes courier cost plus a $10 handling fee. We typically use World Courier to ship live mice and FedEx for other shipments.

◆ Animal Programs

All animal work is conducted in our specific pathogen free (SPF) facilities that have been AAALAC accredited and OLAW assured. For details information, please visit our support section for Description of our Facility, Animal Health and Animal Welfare Program.

◆ Customer References

Please click here to view a map of customer who have used Cyagen before worldwide. 

◆ Citations

Please click here for a list of publications that have cited Cyagen.