TurboKnockout® Gene Targeting Mice

TurboKnockout<sup>®</sup> Gene Targeting Mice
 

The TurboKnockout® gene targeting service is based on traditional embryonic stem cell (ESC)-mediated targeting techniques, which can be used for complex gene modeling projects to provide C57BL/6 or BALB/c mouse models with accurate genetic modification and no off-target effects. Our TurboKnockout® service can provide you with conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months.

 

Cyagen’s TurboKnockout® Gene Targeting Service

This proprietary gene targeting method is enabled by two innovations:

(1) A super competent ES cell line that generates 100% ESC-derived founder mice rather than chimeras, eliminating the need to screen for germline transmission.

(2) A selection cassette on the targeting construct capable of removing itself after ES cell targeting without the need to breed to Flp deleter mice.

These innovations eliminate two generations of breeding, shortening production time by 4-6 months as compared to industry standard. For conditional knockouts, it may be possible to breed founders directly to your tissue-specific Cre mice.

 

Advantages of TurboKnockout® Gene Targeting

Compared with CRISPR/Cas9-based techniques, TurboKnockout® is free of patent disputes, provides precise models on a comparable timeline, and is the technology of choice among our clients performing drug development projects.

  • Freedom to Operate: Technology of choice for drug development projects
  • Success Rate: 100% guaranteed germline transmission (GLT)
  • Turnaround Time: Founders as fast as 6-8 months
  • Large Fragment Knockin: Insert fragments up to 300 kb
  • Flexible Floxing: No size limitations for your floxed region
  • 100% Money-Back Guarantee

 

Comparison of Animal Model Generation Methods

  TurboKnockout® CRISPR/Cas9 Gene Editing Traditional Gene Targeting
Turnaround time 6-8 months 5-7 months 10-14 months
Donor species & backgrounds Mouse: C57BL/6, BALB/c Mouse: C57BL/6, FVB
Rat: Sprague-Dawley (SD), Long Evans
Mouse: C57BL/6, BALB/c
Approach Homologous recombination in ESC by Cyagen’s TurboKnockout® technology CRISPR/Cas9 nuclease mediated gene targeting by pronuclear injection Homologous recombination in ESC by traditional technology
Applications Knockout (KO)
Conditional knockout (cKO)
Point mutation
Reporter knockin (KI)
ROSA26 knockin (KI)
Humanization
Knockout (KO)
Conditional knockout (cKO)
Point Mutation
Limited knockin (KI)
fragment size
Knockout (KO)
Conditional knockout (cKO)
Point mutation
Reporter knockin (KI)
ROSA26 knockin (KI)
Humanization
Knock-in fragment size Up to 300 kb Up to 15 kb (endogenous) Up to 300 kb
Self-deleting selection cassette Yes
No need to breed to Flp deleter mice
N/A No
Requires breeding to Flp deleter mice
Screening method PCR + Southern blot PCR + Sequencing PCR + Southern blot
Patent Risks N/A High-risk N/A

 

Cyagen TurboKnockout® Gene Targeting Mice

>> TurboKnockout® Humanized and Knockin Mice

>> TurboKnockout® Knockout (KO) and Conditional KO (cKO) Mice

 

BALB/c Strain Services Available

Cyagen provides BALB/c mouse models for all embryonic stem cell (ESC) based gene targeting services, including our proprietary TurboKnockout® technology. Over the last several years, Cyagen has tested numerous batches of BALB/c-strain ESCs for key characteristics – including progenicity, karyotype stability, totipotency, reproductive chimerism, and final germline chimerism - to identify BALB/c-derived ESCs suitable for generating targeted genetic models.

Significance of BALB/c Mice in Research

BALB/c is a laboratory-bred, albino strain of the house mouse, characterized by its immunodeficiency and extreme susceptibility to carcinogens. BALB/c mice are among the most widely used mouse strains across oncology, immunology, nuclear medicine research, and are often the first option for animal experimentation models in new drug development projects.

 

Why work with Cyagen?

  • 14+ years' experience in custom animal model generation
  • 50,000+ animal models generated and delivered worldwide
  • 4,000+ citations in SCI journals for our services
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured

 

Why work with Cyagen?

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,700+ citations in SCI journals
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured