Adeno-associated virus (AAV) is a DNA virus that has a single-stranded DNA genome. In contrast, lentiviruses are RNA viruses. Both AAV and Lentivirus are efficient gene delivery systems. They effectively transfer genes into mammalian cells.
Cyagen has many years of experience in virus packaging platforms and can provide AAV, LV, ADV, and other viruses that comply with different quality standards. Our virus packaging platform has been widely used in constructing various cell line models and in vivo studies of living animals. It has been cited and published by customers in many documents. Our experts will work with you to ensure that you receive AAV, lentivirus, and adenovirus packaging services that are customized to your research at every stage, tailored to your needs.
Adeno-associated Virus Packaging
Adeno-associated viruses (AAVs) are promising therapeutic viral vectors. Cyagen can provide adeno-associated virus vectors design and delivery services. For AAV virus packaging, we adopt a three-plasmid co-transfection method. The advanced purification process can provide customized AAV packaging services with high purity, high titer, and different serotypes. It is especially suitable for in vivo animal experiments and can meet the personalized choices of gene therapy researchers.
Lentiviruses are a powerful gene delivery vector that ensures the long-term expression of your target transgene. For Lentivirus packaging, we use 3rd generation lentiviral systems for the highest biosafety standards. Based on our rich experience in virus packaging platforms, Cyagen provides lentivirus (LV) packaging services with different quality standards, which are widely used in the construction of various cell line models, especially for difficult-to-infect T cells used in CAR T-cell construction.
Adenovirus (AdV) is a non-enveloped virus with a diameter of about 90-100nm, which has a wide range of cell and tissue infection capabilities, and the capacity of adenovirus vectors to carry gene fragments can reach 7-8kb. The adenovirus packaging system includes one expression plasmid and one or more helper plasmids. The target gene is expressed independently from the host cell genome, which can achieve rapid and high-abundance expression of the target product while avoiding gene mutation caused by gene integration.
Comparison of Adeno-associated Virus (AAV), Adenovirus (AdV), and Lentivirus (LV) Vectors
|Genome||4.7kb single-stranded DNA or 2.4kb double-stranded DNA||36 kb double-stranded DNA||9.3 kb double-stranded RNA|
|Expression||Phased expression at medium to high level||High-level transient expression||Moderately stable expression|
|Expression Duration||Begin to express within 7-14 days,||Begin to express in 1-2 days,||Begin to express in 2-4 days,|
|Generally 6 months to 24 months||Lasts ≤ (2-4) weeks||Lasts ≥6 months|
|Diffusion capacity in vivo||High||High||Ordinary|
|Integration method||Small amount of targeted integration||No integration||High frequency random integration|
|Advantages||High yield, simple composition, good safety||High yield and easy purification||Low yield, stable expression|
|Application||Animal experiments||Cell/animal larger gene overexpression||Cell experiments|
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