Beyond our Conditional Rat Model Toolkit services, Cyagen’s CRISPR-based Rapid Knockout services provide conventional knockout (KO), reporter knockin (KI), large fragment knockin (LFKI), ROSA26 LFKI, and humanization rat models in as fast as 5 months.
Rat Model Toolkit - Technical Highlights● Mature Gene Editing Platform:Led by internationally renowned Cyagen scientists, Cyagen has successfully delivered over 78,000 custom models to researchers across the globe and received more than 4,800 citations.● Proprietary AI-Based Analysis Tool:Identify the optimal region to target in the gene of interest while minimizing off-target effects.● Unique CRISPR-Pro Technology:CRISPR-Pro technology uses a unique fertilization processing method that enhances the sensitivity of fertilized eggs to pathways involved in homologous recombination (HR)-mediated repair - greatly improving the HR efficiency and positive founder rates.● Diversity of Donor Strains Available:Cyagen not only generates rat models on the Sprague-Dawley (SD) strain background but also generates rat models using Long Evans, Fischer 344, Wistar, and Brown Norway strains.
Cyagen Cre Platform
Despite the utility of the Cre/lox modeling strategy, the complexity of breeding protocol alongside the increased cost of rat colony maintenance has created significant barriers for researchers seeking to make novel conditional rat models. Another major constraint faced by researchers developing cKO or cKI rat models is the lack of Cre lines specific to the target of interest.
Contact us to inquire about your conditional model needs and see how we can help develop a custom Cre strain to support your field of study.