Transgenic Mouse Embryos

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Transgenic Mouse Embryos
We offer a one-stop solution for all your transgenic mouse embryos needs. You can contract us to perform your entire transgenic mouse embryo project from initial strategy design and DNA vector construction to transgenic mouse embryo delivery, or you can outsource specific phases of the project to us. Our turnaround time is the shortest in the industry, our prices are unbeatable, and for guaranteed services, we guarantee the generation of embryos or your money back.
 ◆ Workflow of Transgenic Mouse Embryos Services

Each full transgenic mouse embryos project is split into the following phases:

1. Transgenic strategy design

  • Design a transgenic strategy that meets your research needs.
  • Develop a PCR genotyping strategy for mouse embryos screening.

 

2. Transgenic vector construction

  • Construct conventional plasmid-based transgenic vectors, or modify BACs by recombineering as needed for BAC-based transgenes (visit our DNA Vector Construction & BAC Modification Services page for details).
  • Confirm plasmid or BAC modification by restriction digest and sequencing as needed.

 

3. Pronuclear injection

  • Inject transgenic plasmid or BAC into the pronucleus of fertilized eggs.
  • Implant injected eggs into surrogate mothers to obtain offspring.

 

4. Transgenic mouse embryos screening and LacZ staining

  • Embryos harvested at the desired embryonic stage will be genotyped by PCR and lacZ stained to identify the ones carrying the desired transgene.
Types of Transgenic Mouse Embryos Services

Preparation of DNA for injection and development of a genotyping strategy

The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ a special protocol to prepare top-quality DNA for our injections. Plasmid DNA is typically linearized for injection. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC. A PCR-based genotyping strategy will be developed and tested. If you provide your own transgenic vector rather than having us construct it, you can send us either E. coli or unlinearized DNA in solution (≥50 µg total), and we will perform the necessary preparation to purify/linearize the DNA. We strongly discourage customers from sending us linearized, injection-ready DNA, because the quality of such DNA is often insufficient for pronuclear injection. If you provide your own vector without sequence information, you will also need to provide a PCR-based genotyping strategy along with your vector.

 

Generation of transgenic mouse embryos and staining of embryos for lacZ expression

- Plasmid or BAC injection to obtain embryos, basic service: A minimum of 150 eggs (C57BL/6 strain) will be injected. Embryos will be retrieved from surrogate mothers at the stage you specify (typically within the E10.5-E16.5 window) for subsequent processing. This will result in an average of 2-3 transgenic embryos, but the actual numbers could fluctuate significantly and it is possible that no transgenic embryos are obtained. If you wish to ensure the generation of transgenic embryos, we strongly recommend that you use our guaranteed service option (see below).

 

- Plasmid or BAC injection to obtain embryos, guaranteed service: We guarantee a minimum of 4 transgenic embryos for plasmid injection or 2 transgenic embryos for BAC injection (the actual number of transgenic embryos obtained is typically higher). We will inject as many eggs as needed to fulfill this guarantee.

 

- Genotyping and lacZ staining of embryos: Embryos will be genotyped by PCR. All embryos, regardless of genotype, will also be fixed and stained for lacZ expression.

Donor Strain Information

We typically produce transgenic mice in B6 or FVB strain backgrounds, but we can use other strains per your request. 

Pricing and Turnaround Time

 

Service Donor egg strain Price Turnaround time
Transgenic strategy design   Free 1-4 days
Transgenic vector construction (conventional plasmid or modified BAC)   Please inquire 2-5 weeks
Prepare DNA for injection and develop genotyping strategy   $350 1-2 weeks
Plasmid injection to obtain embryos,
basic service
C57BL/6 $1,950 3-5 weeks
Plasmid injection to obtain embryos, guaranteed service C57BL/6 $2,650 3-7 weeks
BAC injection to obtain embryos,
basic service
C57BL/6 $2,350 3-5 weeks
BAC injection to obtain embryos,
guaranteed service
C57BL/6 $3,950 3-7 weeks
Genotyping and lacZ staining of embryos   $350 1 week
 
Note: For transgenic mouse embryos services not listed above, please inquire about availability and pricing. The turnaround time above does not include the time for obtaining host institution’s approval for mouse importation, nor transit time during shipping.
Guarantee

Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, mobidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.

◆ Inquiries and Quote Requests

Request a quote now. Alternatively, you can always email service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.

Price Matching

If you find another commercial service provider that offers better pricing than ours, we will match the price plus an additional 5% off.

◆ Payments

Standard payment terms include a 50% upfront payment before the project, and the remaining 50% plus shipping charge paid after completion of the project. If you need us to design your transgenic strategy, we will provide this service for free irrespective of whether you end up choosing us for your project. 

Bulk Discount

We offer up to a 10% bulk discount for large orders. Large orders are defined as 5 or more projects from the same institution. If you bundle your orders with those of your colleagues, you can all qualify for the bulk discount. 

Shipping

DNA construct or fixed/stained embryos are shipped at room temperature, and the charge includes courier cost plus a $10 handling fee. We typically use World Courier to ship live mice and FedEx for other shipments.

◆ Animal Programs

All animal work is conducted in our specific pathogen free (SPF) facilities that have been AAALAC accredited and OLAW assured. For details information, please visit our support section for Description of our Facility, Animal Health and Animal Welfare Program.

◆ Customer References

Please click here to view a map of customers who have used Cyagen before worldwide.

◆ Citations

Please click here for a list of publications that have cited Cyagen.