The syngeneic model (also known as allograft mouse tumor systems) involves inoculation of tumor cell lines (derived from the same genetic background strain) which are implanted into the inbred immunocompetent mouse strain. The recipient mouse has an intact native immune system and normal immune response, and its immune system is compatible with the transplanted tumor tissue - which together maximize the simulation of the real-life tumor microenvironment. However, the transplanted mouse tissue may not fully represent the complexity of human tumors in clinical cases.
Cyagen can provide you with various syngeneic models, such as breast cancer, liver cancer, colon cancer, melanoma, lung cancer, and brain cancer models, as well as highly customized in vivo pharmacodynamic services for the corresponding models.
Cyagen can provide various pharmacological evaluation methods, such as tumor growth curve/weight curve, blood biochemical indicators, pathological test (frozen section and paraffin section examination, H&E staining, immunohistochemistry (IHC), etc.), flow cytometry, molecular testing, which can meet your multiple needs for the analysis of drug mechanism.
Backgrounds: Generally C57BL/6 or BALB/c
● Subcutaneous: Convenient to directly observe tumor growth
● In situ: Simulates the original environment of tumor growth
● System injection: Intraperitoneal or intravenous injection to monitor the tumor spread, or metastasis
Selected Syngeneic Model Cases
CT26.WT murine colon carcinoma subcutaneous tumor model
Figure 1. The growth curve of CT26.WT murine colon carcinoma subcutaneous tumor
Figure 2. Formation experiment of CT26.WT cell subcutaneous tumor. CT26.WT cells were inoculated into BALB/c mice through subcutaneous injection, to investigate the tumor volume for different concentrations of cell inoculation.