Type 2 Diabetes Mellitus (T2DM) Mouse Model

Type 2 Diabetes Mellitus (T2DM) Mouse Model

 

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Diabetes Mellitus (DM) has become the third leading chronic disease among the population, following cancer and cardiovascular disease (CVD). This chronic disease is diagnosed when a person does not respond well to insulin or – as the disease progresses - cannot secrete sufficient insulin, resulting in increased glucose levels. Type 2 diabetes mellitus (T2DM) patients account for about 90% of all diabetic patients, and most patients with T2DM experience insulin resistance. The T2DM mouse model can be used in exploring the hypoglycemic effect of drugs on type 2 diabetic mice, which can be prepared by various methods - such as through diet, drugs, surgery, and gene editing.

 

1. Combination of high-fat diet and low-dose streptozotocin-treated mouse model

Construction Strategy: T2DM mouse model was constructed with a high-sugar and high-fat diet fed combined with low-dose intraperitoneal injection of streptozotocin (STZ).

Background: C57BL/6J

Weight: Greater than 18g, the average value is preferably about 20g.

Experimental groups: There are five experimental groups - model control group, positive control group, low-dose group, medium-dose group, and high-dose group.

Testing items: Body weight, food intake, glucose tolerance test (GTT), insulin tolerance test (ITT), fasting blood glucose test, random blood glucose test, serum biochemical test or kit method (ELISA test kit), physiological cage test (urine and feces collection), metabolic cage detection (indicators such as oxygen consumption, heat production, activity, etc.), tissue and organ collection and pathology analysis (H&E, Sirius red, Masson, Oil red, IHC, etc.), etc.

 

Type 2. Diet-induced obesity (DIO) Model

Principles: Continuous high-fat diet (HFD) induced obesity mouse, which can be used for obesity and type 2 diabetes (T2DM) research.

Background: C57BL/6J

Weight: Greater than 18g, the average value is preferably about 20g.

Experimental groups: There are five experimental groups - model control group, positive control group, low-dose group, medium-dose group, and high-dose group.

Test items: Body weight, food intake, glucose tolerance test (GTT), insulin tolerance test (ITT), fasting blood glucose test, random blood glucose test, serum biochemical test or kit method (ELISA test kit), physiological cage test (urine and feces collection), metabolic cage detection (indicators such as oxygen consumption, heat production, activity, etc.), tissue and organ collection and pathology analysis (H&E, Sirius red, Masson, Oil red, IHC, etc.), etc.

 

Type 2 Diabetes - Related genetically engineered mouse models

Gene Backgrounds Storing Status
Abcc8 C57BL/6J Cryopreserved Sperms
Adipoq C57BL/6N Cryopreserved Sperms
Cacna1a C57BL/6J Cryopreserved Sperms
Cacna1b C57BL/6J Cryopreserved Sperms
Cacna1c C57BL/6N Cryopreserved Sperms
Cacna1d C57BL/6N Cryopreserved Sperms
Cacna1e C57BL/6J Cryopreserved Sperms
Cacna1g C57BL/6J Cryopreserved Sperms
Gck C57BL/6J Cryopreserved Sperms
Hk1 C57BL/6J Cryopreserved Sperms
Hk2 C57BL/6J Cryopreserved Sperms
Hk3 C57BL/6N Cryopreserved Sperms
Hkdc1 C57BL/6J Cryopreserved Sperms
Ikbkb C57BL/6J Cryopreserved Sperms
Ins1 C57BL/6J Cryopreserved Sperms
Insr C57BL/6J Cryopreserved Sperms
Irs1 C57BL/6N Cryopreserved Sperms
Irs4 C57BL/6J Cryopreserved Sperms
Kcnj11 C57BL/6J Cryopreserved Sperms
Mafa C57BL/6J Cryopreserved Sperms
Mapk1 C57BL/6J Cryopreserved Sperms
Mapk10 C57BL/6J Cryopreserved Sperms
Mapk3 C57BL/6J Cryopreserved Sperms
Mapk8 C57BL/6J Cryopreserved Sperms
Mapk9 C57BL/6J Cryopreserved Sperms
Mtor C57BL/6J Cryopreserved Sperms
Pdx1 C57BL/6J Cryopreserved Sperms
Pik3ca C57BL/6J Cryopreserved Sperms
Pik3cb C57BL/6J Cryopreserved Sperms
Pik3cd C57BL/6J Cryopreserved Sperms
Pik3r1 C57BL/6J Cryopreserved Sperms
Pik3r2 C57BL/6J Cryopreserved Sperms
Pik3r3 C57BL/6J Cryopreserved Sperms
Pklr C57BL/6J Cryopreserved Sperms
Pkm C57BL/6J Cryopreserved Sperms
Prkcd C57BL/6J Cryopreserved Sperms
Prkce C57BL/6J Cryopreserved Sperms
Prkcz C57BL/6J Cryopreserved Sperms
Slc2a2 C57BL/6J Cryopreserved Sperms
Slc2a4 C57BL/6J Cryopreserved Sperms
Socs1 C57BL/6J Cryopreserved Sperms
Socs2 C57BL/6J Cryopreserved Sperms
Socs3 C57BL/6J Cryopreserved Sperms
Socs4 C57BL/6J Cryopreserved Sperms
Tnf C57BL/6N Cryopreserved Sperms

 

Cyagen can provide you with a variety of drug evaluation models along with  phenotype analysis services – delivering reliable and expedient data reporting for your project.

 

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