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Histology Service

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histology service
With our new histology service, Cyagen can accelerate phenotypic analysis of your mouse and rat models. We now offer histological services including tissue processing, embedding, sectioning and staining using paraffin sectioning. With high quality, reproducible results, our histology allow you to obtain preliminary histology data even before the animals arrive at your facility! We provide histological examination for all major organs/tissues with broad interest, including liver, stomach, small intestine, kidney, testis, ovary, thymus, spleen, heart, pancreas, eye, skin, fat, and muscle. Choose from our basic histology package for standard organs or easily order custom histology services; we also offer customized packages to satisfy your specific research needs.

Workflow of Histology Service

workflow of histology service

1. Sample preparation

We first perform the required breeding needed to obtain transgenic/mutant animals of the desired genotypes. Animals are bred within our SPF facility and sacrificed at specified time points. The tissues/organs are then processed, paraffin embedded, sectioned and stained; For each genotype, a set of tissues will be harvested. Additional samples can be prepared in paraffin blocks upon request.

 

2. Embeding and sectioning

Embedding and sectioning services are based on standard paraffin sectioning protocols. Tissues of interest are first fixed and embedded in paraffin blocks; Paraffin blocks can be used for a variety of downstream analyses, including immunohistochemistry (IHC), in-situ hybridization (ISH), RNA-Seq, PCR, etc. Paraffin embedded tissues can also be preserved in blocks for years with good morphology. Paraffin blocks can be further sliced into thinner sections. We can provide paraffin blocks and the unstained sections. By default, a total of three slides are generated from one organ/tissue sample in this service.

 

For special research purposes like cell lineage tracing that requires serial/step sections, sections collected from different tissue intervals are placed on the same slide to present a full picture of the tissue.

 

3. Hematoxylin and Eosin (H&E) staining

As the most commonly used histologic staining approach, H&E staining provides a detailed view of the tissue for routine examination. While hematoxylin stains nuclei into blue/purple, eosin counterstains cytoplasm, muscle and connective tissue into pink/red.

 

4. Imaging

We currently provide imaging services for the gross morphology and H&E staining. Images will be labeled and stored on our FTP site for download; Data can be accessed anytime and shared with collaborators instantly. We provide three months of FTP data storage for free, and can provide longer storage times upon request.

◆ Pricing and Turnaround Time

Category

Service

Price

Turnaround Time

Custom

histology services

Animal dissection and processing*

$25/mouse**

1-2 weeks

Embedding tissue

$9/block

Sectioning

$12 per 3 slides from the same block***

Sectioning of brain or bone

$20 per 3 slides from the same block***

Step/serial sectioning

Please inquire

H&E staining

$3/slide

Basic histology package

H&E staining for all of the standard tissues****

$270/mouse

1-2 weeks

Other staining

Immunohistochemistry (IHC)

Please inquire

1-2 weeks

In-situ hybridization (ISH)

Please inquire

Handling fee

$18/project

Note: Our fee schedule shown above is for research histology services based on formalin-fixed, paraffin-embedded tissue.

 

Place any animal model generation order and receive up to 25% off histology services.

 

* View the details of breeding service and request a quote.

** The dissection fee will be exempt if there is a request for more than 5 mice.

*** $1.5 for additional slide from the same block.

**** Our current standard tissue list: liver, stomach, colon, small intestine, kidney, testis, ovary, thymus, spleen, heart, eye, lung, skin, fat, and muscle.

◆ Inquiries and Quote Requests

Request a quote now. Alternatively, you can always email service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.

Bulk Discount

We offer up to a 10% bulk discount for large orders. Large orders are defined as 5 or more projects from the same institution. If you bundle your orders with those of your colleagues, you can all qualify for the bulk discount.