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Citations
IMMUNITY 47:107 (2017) IF=22.845
EGFP Reporter Mice
Catalog Number：C001180
Genetic Background：C57BL/6N
Reproduction：Carrier x Carrier
Strain Description
The EGFP protein in EGFP Reporter mice is systemically expressed under the regulation of the CAG promoter, an artificially constructed combinatorial promoter consisting of the cytomegalovirus (CMV) early enhancer element and the chicken beta-actin promoter, which is commonly used to drive high-level expression of genes in expression vectors in mammals.
Transgene (TG) targeting technology was used to insert the EGFP gene expression sequence into the pCAGGS vector, which consists of the chicken β-actin promoter, the CMV early enhancer, the β-actin intron, and the bovine globulin polyA signal.
1. Systemic expression of EGFP in neonatal mice
Figure 1. Green fluorescence throughout the body of a neonatal EGFP Reporter mouse. Systemic green fluorescence expression in this mouse can be observed under excitation light irradiation alone.
2. Expression of EGFP in various tissues
Figure 2. Expression of green fluorescent protein in various tissues of EGFP Reporter mice. Tissues of the heart, liver, spleen, lung, kidney, muscle, testis, intestine, fat and other tissues of EGFP mice were taken and the expression of green fluorescence was observed under the fluorescence microscope. The results showed the presence of green fluorescence in almost all tissues of EGFP Reporter mice.
3. Expression of EGFP in the hair
Figure 3. Expression of EGFP in the hair of EGFP Reporter mice. The results showed that similar to previous studies, there was no expression of EGFP in the hair of EGFP Reporter mice.
This strain was generated using transgenic technology, with the integration site of the EGFP transgene remaining unknown. As a result, conventional PCR can only confirm the presence of the EGFP transgene, but cannot distinguish between homozygous and heterozygous mice.
