Our Experts will contact you providing a quote,
information and estimated timeframe to
your project needs.
Custom Models
-
-
-
-
-
-
-
-
- ● Diet-Induced Obesity (DIO) Mouse Model
- ● Type 1 Diabetes Mellitus (T1DM) Mouse Model
- ● Type 2 Diabetes Mellitus (T2DM) Mouse Model
- ● Diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model
- ● Chemically Induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model
- ● Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Mouse Model (Gene-editing)
- ● Composite (Combined) Model - Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Model
- ● Composite Model - Atherosclerosis Mouse Model
- ● Atherosclerosis Mouse Model (Gene-editing)
- ● Acute Pancreatitis Mouse Model
- ● Chronic Pancreatitis Mouse Model
-
-
-
-
-
-
-
-
-
Testimonials
"I've been very happy with Cyagen's service so I've been referring a lot of colleagues... thanks for
the
great service."
'- Washington University in St. Louis
> more
> more
Citations
IMMUNITY 47:107 (2017) IF=22.845
Sftpc-MerCreMer Mice
Catalog Number: C001501
Stain Name: C57BL/6JCya-Sftpcem2(IRES-MerCreMer)/Cya
Genetic Background: C57BL/6J
Reproduction: Homozygote x Homozygote
Strain Description
The SFTPC gene encodes surfactant protein C (SP-C), one of the four key proteins in surfactant. Surfactant is a mixture of lipids and proteins that coats the lung tissue, making breathing easier. Surfactant is secreted by alveolar cells and maintains the stability of the lung tissue by reducing the surface tension of the fluid that covers the lung. In addition, SP-C is involved in lung development and function, including alveolar septation, airway remodeling, and immune defense. The SFTPC gene is primarily expressed in the lung, with the highest expression in the lower lobe, right lung, upper lobe, left upper lobe, and visceral pleura. It is also expressed at low levels in other tissues. Type II alveolar cells are responsible for the production and secretion of surfactants. Therefore, the SFTPC gene is primarily expressed in these cells and can be used as a specific marker for these cells.
Sftpc-MerCreMer mice were generated to integrate the tamoxifen-inducible MerCreMer recombinase expression element into the 3’UTR of the mouse Sftpc gene. This mimics the expression pattern of the endogenous gene while maintaining Sftpc expression. When bred with mice carrying a loxP site-flanked sequence, Cre recombinase-mediated recombination of the flanked sequence occurs in type II alveolar cells, following tamoxifen induction.
The IRES-MerCreMer gene expression element was integrated into the 3'UTR of the mouse Sftpc gene.
a. Method
Sftpc-MerCreMer mice were mated with Rosa26-LSL-tdTomato mice to generate double heterozygous zygote mice. Tamoxifen induction (4 mg/mouse for 4 days, i.p.) was performed at 6 weeks of age, resulting in Cre recombinase-mediated deletion of the LSL elements and subsequent expression of tdTomato in Cre-positive cells. One week after induction, Lung, trachea, and kidney tissue was collected from the offspring, and the distribution of tdTomato was determined by Immunofluorescent staining to assess Cre recombinase expression. The control group received the same dose of corn oil.
b. Groups
Cre+Tam+: Sftpc-MerCreMer[KI/+];Rosa26-LSL-tdTomato[CKI/+], Tamoxifen-induced;
Cre+Tam-: Sftpc-MerCreMer[KI/+];Rosa26-LSL-tdTomato[CKI/+], Corn oil-treated;
c. Result
1) Expression of Cre recombinase in the lung
Figure 1. Immunofluorescence (IF) staining of lung tissues. Results showed that strong Cre recombinase activity was present in type II alveolar cells of mice induced by tamoxifen (Cre+Tam+). Cre recombinase activity was not detected in the lungs of mice in the non-Tamoxifen-induced group (Cre+Tam-). These results suggest that Cre recombinase activity and specificity in the mouse lung tissue are high, and there is no expression leakage.
2) Expression of Cre recombinase in other tissues
Figure 2. Immunofluorescence (IF) staining of trachea and kidney tissue. Immunofluorescent staining was performed to detect the expression of tdTomato protein in the trachea and kidney tissue of mice. Results showed that Cre recombinase activity was present in a subset of tracheal cells of mice induced by tamoxifen (Cre+Tam+), but not in the kidney. Cre recombinase activity was not detected in these areas of mice in the non-Tamoxifen-induced group (Cre+Tam-). These results suggest that a small amount of Cre recombinase activity is present in a subset of the tracheal tissue of mice.
d. Summary
In the Sftpc-MerCreMer mouse model, Cre recombinase is predominantly expressed in type II alveolar cells. Overall, the mice do not exhibit pre-induction expression leakage. In conclusion, this model is a highly tissue-specific Cre mouse targeting type II alveolar cells.
The Cre recombinase expression element integration site of this strain is located on chromosome 14, please avoid using flox mice with the transgenic locus on chromosome 14 for breeding.
